| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227799 |
| Chromosome: | chromosome 12 |
| Location: | 7973984 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g552150 | (1 of 1) PF00069//PF13185 - Protein kinase domain (Pkinase) // GAF domain (GAF_2) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTGTAGGTGGGTGGTTGTAGGTGGGTGGTT |
| Internal bar code: | TCTAGGGATCGTTTGCATGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 299 |
| LEAP-Seq percent confirming: | 99.1439 |
| LEAP-Seq n confirming: | 11233 |
| LEAP-Seq n nonconfirming: | 97 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGGCTACGCCTTCACTTCTT |
| Suggested primer 2: | AGGTGGGAGAACACTTGTGG |