| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227809 |
| Chromosome: | chromosome 13 |
| Location: | 4180164 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g592200 | GLT1,GSN1 | Glutamate synthase, NADH-dependent; (1 of 1) 1.4.1.14 - Glutamate synthase (NADH) / NADH-glutamate synthase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTGCTTGTGGATGTAGACCTCTCCAAAAG |
| Internal bar code: | CGCCATCATTTATCGAGTACGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 703 |
| LEAP-Seq percent confirming: | 82.0177 |
| LEAP-Seq n confirming: | 5642 |
| LEAP-Seq n nonconfirming: | 1237 |
| LEAP-Seq n unique pos: | 14 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TATGGCGGTGAGACTCTGTG |
| Suggested primer 2: | CTTGCCCCTCGCTACTACTG |