| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227812 |
| Chromosome: | chromosome 16 |
| Location: | 2232225 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g658775 | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTCGTTGTTGTGGTTGTTGTTCCCGCCCTC |
| Internal bar code: | GGGGGTGACAAGAGCTAGGAGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 545 |
| LEAP-Seq percent confirming: | 99.4541 |
| LEAP-Seq n confirming: | 9655 |
| LEAP-Seq n nonconfirming: | 53 |
| LEAP-Seq n unique pos: | 89 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGTATGATAGCCAGGGCG |
| Suggested primer 2: | GAGTTTCCTCCAGTTGCTGC |