| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227849 |
| Chromosome: | chromosome 1 |
| Location: | 4249719 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g028450 | (1 of 1) PTHR30544//PTHR30544:SF2 - 23S RRNA METHYLTRANSFERASE // SUBFAMILY NOT NAMED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GAGTACGTGATGCTGTCGGGCGTCAACGAC |
| Internal bar code: | CCCATTTTACGGCTCTGCTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 297 |
| LEAP-Seq percent confirming: | 90.1316 |
| LEAP-Seq n confirming: | 137 |
| LEAP-Seq n nonconfirming: | 15 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATGCAATAGAGCGAGGAGGA |
| Suggested primer 2: | GACCTCGCATAGAGCTGGTC |