Insertion junction: LMJ.RY0402.227857_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.227857
Chromosome:chromosome 3
Location:3814621
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre03.g170450 (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGCAAGCGCTAGCAGCCACACTCTCTCGTG
Internal bar code:CCCCAGGCGCGTGCAGTTCGC

Confirmation - LEAP-Seq

LEAP-Seq distance:389
LEAP-Seq percent confirming:100.0
LEAP-Seq n confirming:46
LEAP-Seq n nonconfirming:0
LEAP-Seq n unique pos:16

Suggested primers for confirmation by PCR

Suggested primer 1:CTCACACAGCTGTAAGGCCA
Suggested primer 2:CTGGCTGTGCAGTTGTGTTT