| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227876 |
| Chromosome: | chromosome 7 |
| Location: | 467190 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g315550 | (1 of 2) IPR005654//IPR027417 - ATPase, AFG1-like // P-loop containing nucleoside triphosphate hydrolase | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AAAGGGCGGGGGGGGGCGTGCGCTTGGGGC |
| Internal bar code: | ATAAGCGGCTGGGTTACGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 499 |
| LEAP-Seq percent confirming: | 83.5821 |
| LEAP-Seq n confirming: | 56 |
| LEAP-Seq n nonconfirming: | 11 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCAGCCTGCTGCTATCTCA |
| Suggested primer 2: | ACAGCTCACTGCATGTACGG |