| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.227894 |
| Chromosome: | chromosome_2 |
| Location: | 7904299 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre02.g141350 | FAP84,DRC10 | Flagellar Associated Protein | sense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CAAAGACCTTGTTACGCACACGCGCTCAGT |
| Internal bar code: | TGGGTTGTGAAACCCGTTGGCG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 964 |
| LEAP-Seq percent confirming: | 98.8282 |
| LEAP-Seq n confirming: | 16783 |
| LEAP-Seq n nonconfirming: | 199 |
| LEAP-Seq n unique pos: | 24 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAGATCCCTACCTCAAGGGC |
| Suggested primer 2: | CTGTGCTGTGCTATGCCTGT |