Insertion junction: LMJ.RY0402.227909_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.227909
Chromosome:chromosome 5
Location:2938529
Confidence (%):58
Locus systematic id Locus common name Defline Feature
Cre05.g238260 (1 of 1) PF13371//PF13414//PF13432 - Tetratricopeptide repeat (TPR_9) // TPR repeat (TPR_11) // Tetratricopeptide repeat (TPR_16) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGATTGCCACAGGCTGCACCCTCCGCCCGC
Internal bar code:TCTGCAAGCGGCGAACTCCGGT

Confirmation - LEAP-Seq

LEAP-Seq distance:191
LEAP-Seq percent confirming:98.2918
LEAP-Seq n confirming:5409
LEAP-Seq n nonconfirming:94
LEAP-Seq n unique pos:10

Suggested primers for confirmation by PCR

Suggested primer 1:CCCTCTCCTCCTCGCTCTAT
Suggested primer 2:CATACTTGCGTGGCAATGAC