| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.227929 |
| Chromosome: | chromosome 9 |
| Location: | 2784833 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre09.g389050 | CNX5,MAW4 | (1 of 2) PTHR16861//PTHR16861:SF4 - GLYCOPROTEIN 38 // PODOPLANIN; Membrane-associated hydroxyproline-rich glycoprotein 4 | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGATGATGTACTCAAATGTGAAGTTACCG |
| Internal bar code: | TTAGATCGCCTACTGCACTCCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 298 |
| LEAP-Seq percent confirming: | 82.4268 |
| LEAP-Seq n confirming: | 197 |
| LEAP-Seq n nonconfirming: | 42 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTTCTGCATGCTGCTGAGAC |
| Suggested primer 2: | GGCAGTAGCAGGCAGATAGG |