Insertion junction: LMJ.RY0402.227929_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.227929
Chromosome:chromosome 9
Location:2784845
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g389050 CNX5,MAW4 (1 of 2) PTHR16861//PTHR16861:SF4 - GLYCOPROTEIN 38 // PODOPLANIN; Membrane-associated hydroxyproline-rich glycoprotein 4 CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): CGACCTGTCCGCCGCAGTGGTGATGGAGAC
Internal bar code:CGGGAGCTCGCGATCTTCGGAA

Confirmation - LEAP-Seq

LEAP-Seq distance:42
LEAP-Seq percent confirming:10.2004
LEAP-Seq n confirming:56
LEAP-Seq n nonconfirming:493
LEAP-Seq n unique pos:6

Suggested primers for confirmation by PCR

Suggested primer 1:ACTTCACGCTGTTCATGCAG
Suggested primer 2:GGCAGTAGCAGGCAGATAGG