| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.227935 |
| Chromosome: | chromosome 12 |
| Location: | 6943113 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g560450 | (1 of 1) IPR004089//IPR009053 - Methyl-accepting chemotaxis protein (MCP) signalling domain // Prefoldin | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ACCAGCCCAAACGGGGGGTGCGGGAGGCAT |
| Internal bar code: | AGTTGGGACTTATCAGGTGCGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 329 |
| LEAP-Seq percent confirming: | 99.3398 |
| LEAP-Seq n confirming: | 1956 |
| LEAP-Seq n nonconfirming: | 13 |
| LEAP-Seq n unique pos: | 44 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GAATTCAGGAGGGGAGCCTA |
| Suggested primer 2: | CATGTCGTACTGCCTAGCGA |