Insertion junction: LMJ.RY0402.227964_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.227964
Chromosome:chromosome 9
Location:5608452
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre09.g400923 FAP28 Flagellar Associated Protein 28; (1 of 1) PTHR23315//PTHR23315:SF126 - BETA CATENIN-RELATED ARMADILLO REPEAT-CONTAINING // SUBFAMILY NOT NAMED CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TGAAGCGCACACGCCTGCTCACGGCACAGG
Internal bar code:TGGTCCCATGGTCTGCGTAACG

Confirmation - LEAP-Seq

LEAP-Seq distance:1080
LEAP-Seq percent confirming:99.087
LEAP-Seq n confirming:18015
LEAP-Seq n nonconfirming:166
LEAP-Seq n unique pos:29

Suggested primers for confirmation by PCR

Suggested primer 1:GTCGAACTGGTATCGGTCGT
Suggested primer 2:GTCTAATAGGCGCGCTTGAC