| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.227974 |
| Chromosome: | chromosome 7 |
| Location: | 3969953 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g339633 | DNJ20 | (1 of 2) PTHR24078//PTHR24078:SF257 - DNAJ HOMOLOG SUBFAMILY C MEMBER // SUBFAMILY NOT NAMED; DnaJ-like protein | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGCCTGCATGCGCCAGGAGGACGTGCGCTG |
| Internal bar code: | CCGGCCGTACTCTGGCTAGTCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 321 |
| LEAP-Seq percent confirming: | 86.3636 |
| LEAP-Seq n confirming: | 19 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 17 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTTGCTCGTACACACACGC |
| Suggested primer 2: | TCTAGATCCGGGGTCATCAG |