Insertion junction: LMJ.RY0402.227994_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.227994
Chromosome:chromosome 6
Location:4092100
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre06.g278219 SSA6 cilia-sensing, structure and/or assembly; (1 of 239) IPR016024 - Armadillo-type fold 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTTCGGTGCGCTGCGGTCATGTACAGGTAT
Internal bar code:AGGCGTTGAGCATAACAACACC

Confirmation - LEAP-Seq

LEAP-Seq distance:570
LEAP-Seq percent confirming:99.4282
LEAP-Seq n confirming:6434
LEAP-Seq n nonconfirming:37
LEAP-Seq n unique pos:103

Suggested primers for confirmation by PCR

Suggested primer 1:TCGTCTGCAGTCTTCAGTGC
Suggested primer 2:CCTCACCCTAGCTTGTCAGC