| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228015 |
| Chromosome: | chromosome 10 |
| Location: | 354324 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g420450 | (1 of 2) PTHR18841:SF0 - PROTEIN C08G5.3, ISOFORM A-RELATED | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCTGAGAGCACCACACGCCTGAGGGTGTG |
| Internal bar code: | AAATCTGTACGTTTACGCCATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 624 |
| LEAP-Seq percent confirming: | 99.4857 |
| LEAP-Seq n confirming: | 3482 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 15 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACTTCAACCTCCTTCCTGC |
| Suggested primer 2: | TGGTGCATGTGTTGATTGTG |