| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228052 |
| Chromosome: | chromosome 12 |
| Location: | 1493868 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g487400 | (1 of 2) PF03556//PF14555 - Cullin binding (Cullin_binding) // UBA-like domain (UBA_4) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTTGTACGCGGCCGTGTTTGGGGAGGGCAG |
| Internal bar code: | TGAAAAGTGATGACGGGGATCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 559 |
| LEAP-Seq percent confirming: | 99.2255 |
| LEAP-Seq n confirming: | 2306 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 55 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGATGATGCTTGCTGTGTCA |
| Suggested primer 2: | CCATCGGACTTTCCTGTTGT |