Insertion junction: LMJ.RY0402.228053_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.228053
Chromosome:chromosome 7
Location:5986903
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre07.g354600 (1 of 15) 3.4.21.72 - IgA-specific serine endopeptidase / Immunoglobulin A1 protease CDS

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GGTGGCGCCGCCGCCACCGCTACCGCGTCG
Internal bar code:TTCCGGGGGCACATCCAACAAT

Confirmation - LEAP-Seq

LEAP-Seq distance:482
LEAP-Seq percent confirming:99.2754
LEAP-Seq n confirming:137
LEAP-Seq n nonconfirming:1
LEAP-Seq n unique pos:5

Suggested primers for confirmation by PCR

Suggested primer 1:GGAGGAAGATGAGGAGGAGG
Suggested primer 2:CAACAATGGTGGATGAGGTG