| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228056 |
| Chromosome: | chromosome 11 |
| Location: | 3483385 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre11.g481200 | PHX17,PFH6,P4H6 | Prolyl 4-hydroxylase 6; (1 of 5) PTHR10869:SF55 - OXOGLUTARATE/IRON-DEPENDENT OXYGENASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCCACGACTGAAGCGCACCGCTGTGGCAGT |
| Internal bar code: | AGGGGCTACTATTCATGAACAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 579 |
| LEAP-Seq percent confirming: | 97.2553 |
| LEAP-Seq n confirming: | 16477 |
| LEAP-Seq n nonconfirming: | 465 |
| LEAP-Seq n unique pos: | 142 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCCTGGTCAATGTAACCC |
| Suggested primer 2: | GATTCATGTGTGTATGCCGC |