| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228072 |
| Chromosome: | chromosome 17 |
| Location: | 605601 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g700133 | WNK1 | (1 of 5) K08867 - WNK lysine deficient protein kinase [EC:2.7.11.1] (WNK, PRKWNK); WNK protein kinase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AATCCAGCTGTGATGTACGTGACCCCGTAA |
| Internal bar code: | TTCAATTGCTGGTTCAACTACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 0 |
| LEAP-Seq percent confirming: | 1.3468 |
| LEAP-Seq n confirming: | 4 |
| LEAP-Seq n nonconfirming: | 293 |
| LEAP-Seq n unique pos: | 1 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TGCTTACGGGTGGTGTCATA |
| Suggested primer 2: | TTGAACAGCAGGAAGCAATG |