Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.228080 |
Chromosome: | chromosome_9 |
Location: | 4152421 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre09.g393954 | FAP106 | Flagellar Associated Protein | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | TTTCCCCAAGCTTCCTCGCTGCATTTGCCC |
Internal bar code: | TCGCTTTGGCATAGGCCAACCC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 739 |
LEAP-Seq percent confirming: | 99.2942 |
LEAP-Seq n confirming: | 3517 |
LEAP-Seq n nonconfirming: | 25 |
LEAP-Seq n unique pos: | 72 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | CAAGGCCTTAGTTCTGCCTG |
Suggested primer 2: | CTTAAAGCAGCCTTGCAACC |