| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228082 |
| Chromosome: | chromosome 3 |
| Location: | 1998800 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g155950 | (1 of 752) IPR027417 - P-loop containing nucleoside triphosphate hydrolase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CACCGGCATGCAGGCTGGCACGTCACGTCC |
| Internal bar code: | TGGCGTGACGTGGCGGATATGT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 533 |
| LEAP-Seq percent confirming: | 90.5942 |
| LEAP-Seq n confirming: | 3034 |
| LEAP-Seq n nonconfirming: | 315 |
| LEAP-Seq n unique pos: | 22 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATGGATTCACAGGCTTTGG |
| Suggested primer 2: | CCGTTCAAACTAGTGCCCAT |