Insertion junction: LMJ.RY0402.228087_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:+
Strain:LMJ.RY0402.228087
Chromosome:chromosome 7
Location:5523399
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre07.g351100 THB5,PRX (1 of 1) IPR000104//IPR001486//IPR009050 - Antifreeze protein, type I // Truncated hemoglobin // Globin-like; Truncated hemoglobin 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): TTGTCCGTCCTCGGCCAGGAGCAGCACCCC
Internal bar code:CGATTTATCTTACAGGACATGA

Confirmation - LEAP-Seq

LEAP-Seq distance:288
LEAP-Seq percent confirming:99.5708
LEAP-Seq n confirming:2552
LEAP-Seq n nonconfirming:11
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:TCAAGTGACTTCATGGCAGC
Suggested primer 2:TCGTTGTGTGTGAGGTGGAT