| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228094 |
| Chromosome: | chromosome 3 |
| Location: | 3429859 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre03.g167150 | FMO1 | Flavin-containing monooxygenase; (1 of 2) PTHR23023//PTHR23023:SF4 - DIMETHYLANILINE MONOOXYGENASE // FLAVIN-CONTAINING MONOOXYGENASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TTCACAGCCCTCGCGTGATGCCAATCAGCC |
| Internal bar code: | GATTCCGGAGGAACTCGCGATG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 643 |
| LEAP-Seq percent confirming: | 99.5438 |
| LEAP-Seq n confirming: | 1964 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 52 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCACAGCACCTCCTTCTC |
| Suggested primer 2: | TTTGCTGCCTGAACACAAAG |