| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228107 |
| Chromosome: | chromosome 6 |
| Location: | 3344140 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g277500 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GGGCGGGGCGCGCTGTTCACTACTATGCTA |
| Internal bar code: | TGTTACCTAGGGCCGTGCGAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 755 |
| LEAP-Seq percent confirming: | 99.3077 |
| LEAP-Seq n confirming: | 1291 |
| LEAP-Seq n nonconfirming: | 9 |
| LEAP-Seq n unique pos: | 6 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTGATTGAGCACTGCAACG |
| Suggested primer 2: | CATGCAAAAACACGCATACC |