| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228142 |
| Chromosome: | chromosome 7 |
| Location: | 5186099 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre07.g348550 | TGL13 | (1 of 3) PTHR21493:SF153 - PROTEIN T08B1.4, ISOFORM B-RELATED; Putative triacylglycerol lipase | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGCTAGTTGTGCAGCGGCAGTGCGCCAGT |
| Internal bar code: | GGGGTGGTATCTACCGTGGTTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1056 |
| LEAP-Seq percent confirming: | 95.438 |
| LEAP-Seq n confirming: | 2092 |
| LEAP-Seq n nonconfirming: | 100 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CAGTGTAGGGCACTGGAGGT |
| Suggested primer 2: | GCCACCATTCTTGACATCCT |