| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228152 |
| Chromosome: | chromosome 1 |
| Location: | 2013264 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g010848 | (1 of 1) K06997 - uncharacterized protein (K06997) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTAACGCACGCCCCCGCTGCAAGGCGCTAC |
| Internal bar code: | TAGTTTTGGCGGGAACTTTTAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1166 |
| LEAP-Seq percent confirming: | 99.1688 |
| LEAP-Seq n confirming: | 2744 |
| LEAP-Seq n nonconfirming: | 23 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATATTTGCGGCTTTGTTTGG |
| Suggested primer 2: | CTCAAATTTTGCGGCTCTTC |