| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228163 |
| Chromosome: | chromosome 15 |
| Location: | 359773 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre15.g635950 | (1 of 2) PTHR10942 - LEISHMANOLYSIN-LIKE PEPTIDASE | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGTCAGGAAACTCAGCCCCAGCCCCAGCCC |
| Internal bar code: | GGGGTCAAGAATAAAAACGGCT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 7 |
| LEAP-Seq percent confirming: | 78.6517 |
| LEAP-Seq n confirming: | 70 |
| LEAP-Seq n nonconfirming: | 19 |
| LEAP-Seq n unique pos: | 5 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ATCACTTGCAACTAGCGCCT |
| Suggested primer 2: | CACTGACCTCGCCACACTAA |