Insertion junction: LMJ.RY0402.228166_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.228166
Chromosome:chromosome 6
Location:8021259
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g304100 (1 of 1) K17261 - adenylyl cyclase-associated protein (CAP1_2, SRV2) intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCCCAACAATGTAAACCCCGCCCGCCCCC
Internal bar code:AGCCTACCCAAGATTCCGTGGA

Confirmation - LEAP-Seq

LEAP-Seq distance:404
LEAP-Seq percent confirming:84.375
LEAP-Seq n confirming:81
LEAP-Seq n nonconfirming:15
LEAP-Seq n unique pos:22

Suggested primers for confirmation by PCR

Suggested primer 1:GGGATGTGAGCAAGCTCTTC
Suggested primer 2:CAATTTCCTTCTGCAGCCTC