| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228166 |
| Chromosome: | chromosome 17 |
| Location: | 5652062 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre17.g738350 | PGA5,LPAAT2 | ER 1-acyl-sn-glycerol-3-phosphate acyltransferase; (1 of 2) PTHR10434 - 1-ACYL-SN-GLYCEROL-3-PHOSPHATE ACYLTRANSFERASE | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCTTAGTATCTCTCCGTGCGTGCGGGTGCG |
| Internal bar code: | CAGGTGAGAGACAGGTAACGTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 613 |
| LEAP-Seq percent confirming: | 97.0071 |
| LEAP-Seq n confirming: | 7001 |
| LEAP-Seq n nonconfirming: | 216 |
| LEAP-Seq n unique pos: | 90 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTCCTGTGAATGCCTGGTT |
| Suggested primer 2: | CACGTGTCGTTGTTGTTTCC |