Insertion junction: LMJ.RY0402.228171_2

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:+
Strain:LMJ.RY0402.228171
Chromosome:chromosome 4
Location:2861078
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre04.g224400 (1 of 1) 3.6.3.25//3.6.3.44 - Sulfate-transporting ATPase // Xenobiotic-transporting ATPase / Steroid-transporting ATPase intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): ACCTGGCCCTGCGCGCGCGCGAGCACCACC
Internal bar code:GGAATCGCTGGAGTAGTCACTG

Confirmation - LEAP-Seq

LEAP-Seq distance:325
LEAP-Seq percent confirming:95.8115
LEAP-Seq n confirming:183
LEAP-Seq n nonconfirming:8
LEAP-Seq n unique pos:2

Suggested primers for confirmation by PCR

Suggested primer 1:CTCCGCATTTCTATTCCTGC
Suggested primer 2:GTGATCTCCAGCAGGAAGGA