| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228185 |
| Chromosome: | chromosome 16 |
| Location: | 3853668 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre16.g689950 | (1 of 1) PF00168//PF08372 - C2 domain (C2) // Plant phosphoribosyltransferase C-terminal (PRT_C) | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | TGGAACGCCAGCACGGCCTGGGGGTGCATG |
| Internal bar code: | GCTGGAGTGCGCTAACAGGGTT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 546 |
| LEAP-Seq percent confirming: | 99.3421 |
| LEAP-Seq n confirming: | 755 |
| LEAP-Seq n nonconfirming: | 5 |
| LEAP-Seq n unique pos: | 31 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGTTGGCACCTCCATACCAG |
| Suggested primer 2: | CTTCGTGCCTTGACCTTTTC |