| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228192 |
| Chromosome: | chromosome 6 |
| Location: | 5469893 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g284550 | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTGCGTCCGCACCCACCTGGCAACCCCC |
| Internal bar code: | TTTGCGAATGAAAACTGAAGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 254 |
| LEAP-Seq percent confirming: | 89.4358 |
| LEAP-Seq n confirming: | 745 |
| LEAP-Seq n nonconfirming: | 88 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CTCCTCAAAGTCAAGGTCGG |
| Suggested primer 2: | CATGCTCTTCCAACCATCCT |