| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228210 |
| Chromosome: | chromosome 6 |
| Location: | 5234422 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g282200 | SNF7,VPS32 | Subunit of the ESCRT-III complex, SNF homolog | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GCGGCGCATGCAGTGTGTGGGCGTAAGGAG |
| Internal bar code: | CACTTAAGGGGGCTCGAGAGAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 611 |
| LEAP-Seq percent confirming: | 98.5957 |
| LEAP-Seq n confirming: | 4704 |
| LEAP-Seq n nonconfirming: | 67 |
| LEAP-Seq n unique pos: | 42 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGCTGCTAGAGAAGCGTAT |
| Suggested primer 2: | GGAGGCAGGAAGGAGCTAGT |