| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228217 |
| Chromosome: | chromosome 2 |
| Location: | 5467974 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre02.g107850 | MOB1 | (1 of 1) K06685 - MOB kinase activator 1 (MOB1, Mats); Cytokinesis-related protein | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTGGAAAGGGGCGCACACAACCAACCTTGC |
| Internal bar code: | CGGCGAGGTAGGGCCAGGCGGA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 640 |
| LEAP-Seq percent confirming: | 99.2198 |
| LEAP-Seq n confirming: | 3052 |
| LEAP-Seq n nonconfirming: | 24 |
| LEAP-Seq n unique pos: | 68 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACTCAACTGGGCAATAACGG |
| Suggested primer 2: | CCTCATCGATGTTGCTCTGA |