Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.228223 |
Chromosome: | chromosome 2 |
Location: | 2844274 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Feature |
---|---|---|---|
Cre02.g094650 | (1 of 1) PTHR11702:SF31 - MITOCHONDRIAL RIBOSOME-ASSOCIATED GTPASE 2 | CDS |
Insertion site details | |
Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGTTTGGTCCCACAGCCGGCGCCTTTCTCT |
Internal bar code: | ACGGGCTAGTGAGCCGATCCAT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 729 |
LEAP-Seq percent confirming: | 82.9234 |
LEAP-Seq n confirming: | 1787 |
LEAP-Seq n nonconfirming: | 368 |
LEAP-Seq n unique pos: | 40 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TCGCTGTGCGTATATTGAGC |
Suggested primer 2: | GTTGATCGAATCCTTGCCAT |