| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228226 |
| Chromosome: | chromosome 10 |
| Location: | 5511335 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre10.g459200 | PMA2,ACA4 | (1 of 3) K01535 - H+-transporting ATPase (E3.6.3.6); P-type ATPase/cation transporter, plasma membrane | 5'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CCAAATGTGACCGCCGAGAGTCGCCTAAGT |
| Internal bar code: | GACGACCCATTGGGTGTAATTG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1117 |
| LEAP-Seq percent confirming: | 99.1853 |
| LEAP-Seq n confirming: | 3774 |
| LEAP-Seq n nonconfirming: | 31 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AATAGTGGAGGTGGGTGCTG |
| Suggested primer 2: | ACATGTAGCCGAGGAACACC |