| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228234 |
| Chromosome: | chromosome 12 |
| Location: | 1715583 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g512750 | TEF20 | Notchless-like protein; (1 of 1) PTHR22847//PTHR22847:SF50 - WD40 REPEAT PROTEIN // SUBFAMILY NOT NAMED | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATTTCCAACGGTTGGAGGCGGGCCGGGCAG |
| Internal bar code: | AAAGCGGACGCCAAGCGCAATC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 762 |
| LEAP-Seq percent confirming: | 99.3927 |
| LEAP-Seq n confirming: | 5728 |
| LEAP-Seq n nonconfirming: | 35 |
| LEAP-Seq n unique pos: | 12 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACACAAAATAAAGTGGCGGC |
| Suggested primer 2: | CACATTTACCACCACCACCA |