Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | + |
Strain: | LMJ.RY0402.228247 |
Chromosome: | chromosome_1 |
Location: | 3935538 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre01.g025700 | antisense | intron |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | CGAAGTGCAGGTAACTCACCAGTCGAGCAG |
Internal bar code: | CTCCGCCGTAAGGTATCCCCAC |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 490 |
LEAP-Seq percent confirming: | 91.4943 |
LEAP-Seq n confirming: | 398 |
LEAP-Seq n nonconfirming: | 37 |
LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | GTTATTGCTGCTGCGGCTAC |
Suggested primer 2: | TCCCTAAAGTCACAGCGGTC |