| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228247 |
| Chromosome: | chromosome 1 |
| Location: | 3935538 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre01.g025700 | (1 of 8) PF00211//PF01547 - Adenylate and Guanylate cyclase catalytic domain (Guanylate_cyc) // Bacterial extracellular solute-binding protein (SBP_bac_1) | intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CGAAGTGCAGGTAACTCACCAGTCGAGCAG |
| Internal bar code: | CTCCGCCGTAAGGTATCCCCAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 490 |
| LEAP-Seq percent confirming: | 91.4943 |
| LEAP-Seq n confirming: | 398 |
| LEAP-Seq n nonconfirming: | 37 |
| LEAP-Seq n unique pos: | 18 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GTTATTGCTGCTGCGGCTAC |
| Suggested primer 2: | TCCCTAAAGTCACAGCGGTC |