| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228253 |
| Chromosome: | chromosome 13 |
| Location: | 4469704 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre13.g603550 | 5'UTR_intron |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | ATACGACACGGCGTGGCTTCATTCCGTCCA |
| Internal bar code: | ACGTCGACCGAACGGCAAGGGC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 543 |
| LEAP-Seq percent confirming: | 94.4882 |
| LEAP-Seq n confirming: | 240 |
| LEAP-Seq n nonconfirming: | 14 |
| LEAP-Seq n unique pos: | 7 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AAAACCTGCTCCAGAGACGA |
| Suggested primer 2: | GTTAGCAGCGCAGTAGTCCC |