| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228262 |
| Chromosome: | chromosome_9 |
| Location: | 3879489 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre09.g392060 | ZIP4 | Zinc/iron transporter | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GCCTGCACCCGTGCCTGTTGCTGAGGGGCA |
| Internal bar code: | GACACGCCTGCCATGGGTTTGG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 201 |
| LEAP-Seq percent confirming: | 91.922 |
| LEAP-Seq n confirming: | 330 |
| LEAP-Seq n nonconfirming: | 29 |
| LEAP-Seq n unique pos: | 19 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCGTCAACAGACCACTCAGA |
| Suggested primer 2: | GCGATCCTCTCTTGATCCTG |