| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | - |
| Strain: | LMJ.RY0402.228266 |
| Chromosome: | chromosome 6 |
| Location: | 2964413 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre06.g273200 | (1 of 1) K03762 - MFS transporter, MHS family, proline/betaine transporter (proP) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | GTGTTAGAAGTGACCAGGCCACTTTGGCAA |
| Internal bar code: | AGTCATGAACATGAAACAGACG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 846 |
| LEAP-Seq percent confirming: | 99.0092 |
| LEAP-Seq n confirming: | 4397 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 84 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | ACATGTTCCTGTACCTCGCC |
| Suggested primer 2: | GGCTGTAGTGTCTCCTTGGC |