Insertion junction: LMJ.RY0402.228266_2

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.228266
Chromosome:chromosome 6
Location:2964417
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre06.g273200 (1 of 1) K03762 - MFS transporter, MHS family, proline/betaine transporter (proP) 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTCCTTCCTTAACAGCCCTGCACATGTTGG
Internal bar code:GACAAGTGCGCAGGCTCTGTTC

Confirmation - LEAP-Seq

LEAP-Seq distance:948
LEAP-Seq percent confirming:99.6462
LEAP-Seq n confirming:845
LEAP-Seq n nonconfirming:3
LEAP-Seq n unique pos:13

Suggested primers for confirmation by PCR

Suggested primer 1:ACATGTTCCTGTACCTCGCC
Suggested primer 2:ACCGGTATACCAGCTGTTGC