| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228289 |
| Chromosome: | chromosome_12 |
| Location: | 4419925 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre12.g521050 | antisense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCGCACAGACATCCCACTTCACGGCGTCG |
| Internal bar code: | CCGATTGCCGCACTCTGAACAA |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 658 |
| LEAP-Seq percent confirming: | 99.2228 |
| LEAP-Seq n confirming: | 2298 |
| LEAP-Seq n nonconfirming: | 18 |
| LEAP-Seq n unique pos: | 26 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GCTGTCCTGATGTCGTCTGA |
| Suggested primer 2: | CTTGAGAAGCGGGATCTGAC |