Insertion cassette: | CIB1 |
Side of cassette: | 3' |
Strand: | - |
Strain: | LMJ.RY0402.228314 |
Chromosome: | chromosome_4 |
Location: | 3763997 |
Confidence (%): | 95 |
Locus systematic id | Locus common name | Defline | Orientation | Feature |
---|---|---|---|---|
Cre04.g229494 | antisense | 3'UTR |
Insertion site details | |
Flanking sequence (orientation from cassette outwards): | AAGGCACAAGAGCATGCGCCCGATGCGGTG |
Internal bar code: | CGCCATAGTCCAATAGGTATCT |
Confirmation - LEAP-Seq | |
LEAP-Seq distance: | 651 |
LEAP-Seq percent confirming: | 99.5169 |
LEAP-Seq n confirming: | 10093 |
LEAP-Seq n nonconfirming: | 49 |
LEAP-Seq n unique pos: | 152 |
Suggested primers for confirmation by PCR | |
Suggested primer 1: | TGCTTAGACCAGCGAACCTT |
Suggested primer 2: | CCGTGTGTGTGTGTGTGTGT |