Insertion junction: LMJ.RY0402.228314_1

Overview

Insertion cassette:CIB1
Side of cassette:3'
Strand:-
Strain:LMJ.RY0402.228314
Chromosome:chromosome 4
Location:3763997
Confidence (%):95
Locus systematic id Locus common name Defline Feature
Cre04.g229494 (1 of 70) 3.1.3.16 - Protein-serine/threonine phosphatase / Serine/threonine specific protein phosphatase 3'UTR

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): AAGGCACAAGAGCATGCGCCCGATGCGGTG
Internal bar code:CGCCATAGTCCAATAGGTATCT

Confirmation - LEAP-Seq

LEAP-Seq distance:651
LEAP-Seq percent confirming:99.5169
LEAP-Seq n confirming:10093
LEAP-Seq n nonconfirming:49
LEAP-Seq n unique pos:152

Suggested primers for confirmation by PCR

Suggested primer 1:TGCTTAGACCAGCGAACCTT
Suggested primer 2:CCGTGTGTGTGTGTGTGTGT