| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228317 |
| Chromosome: | chromosome_11 |
| Location: | 2506771 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre11.g475850 | ZNJ1 | DnaJ-like Zinc-finger protein | antisense | intron |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | CCGGAAGGCTTGACGCCCTACTAGAGCCGC |
| Internal bar code: | CGCGATGGAGATCTGTGTCAAC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 565 |
| LEAP-Seq percent confirming: | 99.115 |
| LEAP-Seq n confirming: | 896 |
| LEAP-Seq n nonconfirming: | 8 |
| LEAP-Seq n unique pos: | 21 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CACCAGTCCTGCCTAAGCTC |
| Suggested primer 2: | CCTTTAATTTTGCGCCGTTA |