| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228324 |
| Chromosome: | chromosome 12 |
| Location: | 8266405 |
| Confidence (%): | 73 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g550250 | (1 of 3) PF03638 - Tesmin/TSO1-like CXC domain, cysteine-rich domain (TCR) | 3'UTR |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | CTCTGAAAGGACCCCGCCATGAACTGCCTA |
| Internal bar code: | CAGCGGCAATCGCCCTAGAAAG |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 828 |
| LEAP-Seq percent confirming: | 99.5123 |
| LEAP-Seq n confirming: | 8978 |
| LEAP-Seq n nonconfirming: | 44 |
| LEAP-Seq n unique pos: | 11 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | GGGAGAGGGAGTTGGTAAGG |
| Suggested primer 2: | CTAGTGGAGAGGAAGCCACG |