| Insertion cassette: | CIB1 |
| Side of cassette: | 3' |
| Strand: | + |
| Strain: | LMJ.RY0402.228345 |
| Chromosome: | chromosome_8 |
| Location: | 4541123 |
| Confidence (%): | 58 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre08.g382700 | UBQ3 | Bi-ubiquitin | sense | 3'UTR |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | ATCAGGAAAAGGGGGCGGGGAAGAGGTAGG |
| Internal bar code: | AGTGGCAAGTAGATAAAAAATT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 6 |
| LEAP-Seq percent confirming: | 94.3396 |
| LEAP-Seq n confirming: | 50 |
| LEAP-Seq n nonconfirming: | 3 |
| LEAP-Seq n unique pos: | 2 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | CGCACTTGCCGGTATTTACT |
| Suggested primer 2: | TTCTGGCAACAGAGCATACG |