| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | + |
| Strain: | LMJ.RY0402.228350 |
| Chromosome: | chromosome 12 |
| Location: | 1899516 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Feature |
|---|---|---|---|
| Cre12.g511100 | (1 of 1) IPR000104//IPR007379 - Antifreeze protein, type I // Tim44-like domain | CDS |
Insertion site details | |
| Expected flanking sequence (starting at cassette; orientation from cassette outwards): | AGGCGCTGGTGGCGGGCGCGTCCGTCTTCG |
| Internal bar code: | GGTATTGAGCACGGGATTGCCC |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 1086 |
| LEAP-Seq percent confirming: | 99.1785 |
| LEAP-Seq n confirming: | 28735 |
| LEAP-Seq n nonconfirming: | 238 |
| LEAP-Seq n unique pos: | 29 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | AGGTTGGTGATGATGGTGGT |
| Suggested primer 2: | CGAACGATCCGTATTGGAGT |