| Insertion cassette: | CIB1 |
| Side of cassette: | 5' |
| Strand: | - |
| Strain: | LMJ.RY0402.228365 |
| Chromosome: | chromosome_6 |
| Location: | 3683016 |
| Confidence (%): | 95 |
| Locus systematic id | Locus common name | Defline | Orientation | Feature |
|---|---|---|---|---|
| Cre06.g278139 | Small Rab-related GTPase | sense | CDS |
Insertion site details | |
| Flanking sequence (orientation from cassette outwards): | GTCAGCTTCACGCGCTTGTCGTACACGCTC |
| Internal bar code: | ATCGCGTGACACGATATCGTAT |
Confirmation - LEAP-Seq | |
| LEAP-Seq distance: | 538 |
| LEAP-Seq percent confirming: | 11.0087 |
| LEAP-Seq n confirming: | 681 |
| LEAP-Seq n nonconfirming: | 5505 |
| LEAP-Seq n unique pos: | 3 |
Suggested primers for confirmation by PCR | |
| Suggested primer 1: | TTCGGTACCACATACAGGCA |
| Suggested primer 2: | TGGAGCATGAGAGGACACAG |