Insertion junction: LMJ.RY0402.228369_1

Overview

Insertion cassette:CIB1
Side of cassette:5'
Strand:-
Strain:LMJ.RY0402.228369
Chromosome:chromosome 10
Location:2522085
Confidence (%):73
Locus systematic id Locus common name Defline Feature
Cre10.g436500 XPP SurE-like phosphatase/nucleotidase; (1 of 1) PTHR30457:SF0 - 5'/3'-NUCLEOTIDASE SURE intron

Insertion site details

Expected flanking sequence (starting at cassette; orientation from cassette outwards): GTAAACAGTCGCGCTGCAACGAGAAACTGC
Internal bar code:TTCTTCCAAAGATAGTAACATA

Confirmation - LEAP-Seq

LEAP-Seq distance:628
LEAP-Seq percent confirming:99.2993
LEAP-Seq n confirming:992
LEAP-Seq n nonconfirming:7
LEAP-Seq n unique pos:8

Suggested primers for confirmation by PCR

Suggested primer 1:TAGGTTGATCGAAACTGGGG
Suggested primer 2:TCAGCGCAGCAAACATAATC